10.1107/s205225251500202x 612 TripathiT.UverskyV
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This work was partially supported by the National Natural Science Foundation (31770796 to Y.J., 81730099 to X.X.), the National Science and Technology Major Project (2018ZX09711002-002-002) to Y.J., the National Key R&D Programs of China (2018YFA0507002), the Shanghai Municipal Science and Technology Major Project (2019SHZDZX02), the CAS Strategic Priority Research Program (XDB37030103) to H.E.X., and the Shanghai Municipal Science and Technology Commission Grant (20S11903200) to X.X
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To preserve and stabilize enzymes in formulation, technologies have been developed, among them encapsulation using liposome or nanoemulsion, addition of sugar or polyol as cosolute strengthening the hydration shell around the enzyme, or precise pH control matching the isoelectric point ensuring structural stability [193, 194]
