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The soluble fraction was clarified by centrifugation (75,000 g , 20 min, 4 C) and incubated with 50% Ni-Sepharose suspension (GE Healthcare, Chalfont St Giles, UK) for 2 h at 4 C with gentle rotation prior to affinity purification

The peptide has recently been the subject of much medical research and is becoming increasingly popular among athletes and bodybuilders as a performance enhancer

J Endocrinol Invest 41:583590

0.03) mg/L ( p = 0.01) but not by metformin/placebo, and with no significant difference between treatments

Urocortins in the mammalian endocrine system

This supports overall fat loss (including around the waist)