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severe hypoglycemia occurred in 0.8% and 1.2% of patients when semaglutide 0.5 mg and 1 mg, respectively, was given with a sulfonylurea

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10.3389/fpubh.2022.996179 49 SmitsM

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Quantitative PCR Total RNA was isolated from MIN6 cells using RNAiso Plus reagent (Takara Bio Inc., Shiga, Japan), and 2-g aliquots were converted into cDNA using an Applied Biosystems High Capacity cDNA Reverse Transcription Kit (Life Technologies Co, Carlsbad, CA)