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Cigarette smoke extract (CSE) preparation CSE was prepared by burning one 3R4F reference cigarette, bubbling the direct and side-stream smoke into a 50 ml conical tube containing 25 ml of DMEM/F12 (Gibco, Cat#11320-033) without FBS and antibiotics using a peristaltic pump (Precision Blood Pump, COBE Perfusion System) at 0.12 rpm

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Packaging and Labeling Rules Health warnings may occupy a larger portion of packaging, while color, gloss, and imagery could face tighter restrictions