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LMS-1 washing and LMS-1 recycling An antioxidant solution was prepared by combining 0.75 mM glutathione, 0.75 mM ascorbic acid, and 0.75 mM proline, following the method outlined by Arabidopsis growth conditions Seeds for Col-0 were obtained from The Arabidopsis Biological Resource Center (ABRC) at Ohio State University, stock CS28994