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U-100 contains 100 units of insulin per mL

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Proteins were then separated via SDS-PAGE and transferred to PVDF membranes, which were blocked with 5% skim milk for 1 h at 25 C before incubation with primary antibodies for 12 h at 4 C

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Envelopment efficiency and release rate of GLP-1RAs from GLP-1RAs@NPs High-performance liquid chromatography (HPLC) was performed to measure the encapsulation efficiency and release rate

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