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Then, the sections were coated with an anti-CD31 antibody (Santa Cruz Biotechnology, Dallas, TX) at 4 C overnight, followed by incubation with an HRP-labeled secondary antibody (Gene Tech, Shanghai, China), and the sections were visualized with a DAB system (Gene Tech)

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After 15 days of administration, patients exhibited notable improvements in skin hydration, elasticity, pruritus, and sleep disturbances