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Illien-Junger S, Gantenbein-Ritter B, Grad S, Lezuo P, Ferguson SJ, Alini M, Ito K: The combined effects of limited nutrition and high-frequency loading on intervertebral discs with endplates

THP-1 cells were seeded at 2 10 5 cells per well in complete RPMI 1640 medium with 100 nM phorbol 12-myristate 13-acetate (PMA) (Cayman) and differentiated into macrophages for 3 days, then rested in PMA-free medium for 16 h before infection

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They also aim to support the bodys ability to produce, recycle, and maintain healthy glutathione activity within cells

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