doi: 10.1038/s41419-018-0476-2
I was wondering whether the content of those vesicles could also serve the macrophage metabolism. Now, Garaude and his team have found that macrophages salvage nutrients from phagocytosed bacteria to support their energy requirements, and that this metabolic recycling differs between dead and live bacteria
It's essential to note that while these benefits are promising, individual responses to supplementation can vary
Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)
Glutathione is a tripeptide antioxidant synthesised in every cell of the body, involved in neutralising free radicals and supporting detoxification pathways
Night dosing misses this metabolic alignment