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The cells were rinsed with PBS twice to remove excessive JC-1 dye and the FLUOstar Omega microplate reader was used to measure the fluorescence intensity of JC-1 monomers (488 nm/530 nm of excitation/emission) and aggregates (530 nm/590 nm of excitation/emission)

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These subclusters exhibited the highest expression of ARX and DNMT1 , suggesting that these subpopulations represent the most mature cell subclusters (Figure 3E

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