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To generate endothelial cell-specific Pcsk5 knockout mice on a C57BL/6 background ( Pcsk5 ECKO ), Pcsk5 loxP mice were crossed with the Cdh5-2A-CreERT2 line

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To ensure rigor, myofibrils isolated from one control and one treated heart were analysed on the same day with blinding to the experimental groups

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