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2.3 Urine sample preparation 2.3.1 Sample preparation for acylcarnitine analysis Quality control (QC) and patient urine samples were prepared by adding 100 L of an acylcarnitine isotope working solution (Table S1) and 30 L of ACN to 10 L of each sample in a microcentrifuge tube

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Male C57BL/6N mice were fed a 60% fat diet from 9 weeks of age until they became obese (body weight 40 g at 15-21-week old

To ease discomfort, apply ice for 10 to 15 minutes in the first 24 hours to reduce swelling, then switch to heat to promote blood reabsorption

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