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It represents the zone where peptide bond stability remains high while ice crystal formation (which occurs below 0C) is prevented

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Covert hepatic encephalopathy consists of minimal and grade 1 hepatic encephalopathy 1 , could impair the cognitive function, lower work efficiency, reduce the quality of life 6,7 , impair driving skills 8,9,10,11 , or results in poor prognosis 12,13,14

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