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The lysate was carefully mixed for 10 minutes at 4 C and then centrifuged at 16,000 RCF for 30 minutes at 4 C

TMAO can also promote endoplasmic reticulum stress (ERS) by up-regulating SR-1 receptors, while downregulating ATP-binding cassette transporter A1 in macrophages (64)

However, whether the modulation of autophagy is directly related to RGC survival in our study requires further investigation

Briefly, whole blood (in vivo study) or RBC suspension (ex vivo experiments) was diluted 5X in ice cold 5% Trichloroacetic acid (TCA) solution and deproteinized during a 30min incubation on ice

doi:10.1016/S0361-9230(99)00072-6