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2.12.3 Intrinsic fluorescence Depending on their origin, fluorophores are classified as intrinsic (occurring naturally in the system under study) and extrinsic (fluorophores that do not occur in nature), which are further subdivided to fluorescent dyes (fluorophores that can interact with biomolecules but are not covalently attached to them) and fluorescent labels/probes/tags (fluorophores that are covalently attached to the biomolecules)

One exception is regular B12 supplementation

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If you added 1mL instead of 2mL, your concentration is 5mg/mL, double what you calculated for