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How IGF-1 LR3 Works Reduced IGFBP binding The N-terminal extension and R3 substitution disrupt binding to IGF-binding proteins, keeping LR3 free in plasma rather than sequestered

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2.5 Reverse transcription and real-time quantitative PCR Total RNA was extracted by using RNAiso Plus reagent (Takara, 9109) following the protocol recommended by the manufacturer