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Cell culture INS-1 832/3 cells with endogenous rat Glp1r deleted by CRISPR/Cas9 (11) (referred to as INS-1 832/3 GLP-1R KO cells, kind gift from Dr Jacqueline Naylor, Astra Zeneca) were derived from parental male rat insulinoma INS-1 832/3 cells (Prof Christopher Newgard, Duke University, USA)

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