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The shortage cannot be offset by groundwater supplies, a third of which are already in distress

doi: 10.3389/fonc.2025.1719528 179 AlotaibiSAGareeballahASami Ur RahmanJFaqihiWAAliSElbashirMet al

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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST