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Proteins were then separated via 8% SDS-PAGE (Servicebio, Wuhan, China), transferred to PVDF membranes, and blots were then blocked for 2 h at room temperature with 5% non-fat milk prior to incubation overnight with appropriate primary antibodies at 4C

Salehi S, Naghib SM, Garshasbi HR, Ghorbanzadeh S, Zhang W

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This deactivation of MKPs allows for the unimpeded progression and sustained activation of the pro-inflammatory ERK1/2, JNK, and p38 signaling cascade within the MAPK pathway ( Astrocytes, the major glial support cells in the CNS, are indispensable in the regulation of GSH homeostasis