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A sensible experiment spreads doses across at least three orders of magnitude, something like 1, 10, and 100 nanomolar on up to 1 and 10 micromolar, precisely because the sweet spot moves with the copper-to-peptide ratio, the buffer, and how much serum sits in the media

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Briefly, 100 L of plasma was mixed with three parts ethanol

Vanommeslaeghe, K., Raman, E

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The pineal gland contributes to circadian timing through the synthesis and release of melatonin