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To distinguish malignant tumor cells from normal epithelial cells, we applied a normal hepatocyte gene signature (Supplementary Fig

and cell survival via HIF-1 induction

After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

Side effects, particularly nausea, are more pronounced than single-agent therapy but manageable with proper strategies

However, studies that address all three aspects of the gutimmunebrain axis simultaneously remain limited

Common adverse reactions include gastritis-like symptoms and bleeding