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Take 15 to 30 seconds for the full volume

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The powder was suspended in 2 ml of histone lysis buffer (10 mM Hepes [pH 7.9], 1.5 mM MgCl 2 , 10 mM KCl in Milli-Q H 2 O with the addition of 0.6 mM DTT, sodium butyrate, and 1x cOmplete protease inhibitor EDTA-free immediately before use) and incubated on a roller for 30 min at 4C

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