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For the quantification of sulfur metabolites in the tissue samples, the tissue metabolites were extracted and derivatized with NEM in ice-cold extraction solvent (80% MeOH: 20% H 2 O containing 25 mM NEM and 10 mM ammonium formate, pH=7.0) which includes stable isotope labeled internal standards (20 M [ 13 C 3 , 15 N]-cysteine-NEM, 36.4 M [ 13 C 2 , 15 N]-GSH-NEM, 10 M [D 4 ]-Cystine, 0.92 M [ 13 C 5 , 15 N 2 ]-GSSG, 20 M [D 4 ]-Hypotaurine, and 20 M of [ 13 C 2 ]-Taurine at a final concentration of 50 mg/mL followed by incubation on 4 C for 24 hr

Anal Biochem

Interestingly, a recent study has shown that xylitol, a compound found in the fungus Cordyceps militaris , may be a promising anticancer therapy via regulation of GSH [80]

The Deeper Health Risks The link between this genetic trait and major health problems is well-established by now

(2008) 49(Suppl 8):35

[DOI] [PubMed] [Google Scholar] Cobbett C.S., May M.J., Howden R., Rolls B