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Zepbound works by activating receptors for two gut hormones, GLP-1 (glucagon-like peptide-1) and GIP (glucose-dependent insulinotropic polypeptide)

A lactam bridge loaded at Glu 22 , either c[Lys 18 Glu 22 ] or c[Glu 22 Lys 26 ], shows lower affinity and activity than a simple substitution of Gly 22 with Glu