Cells were washed three times with PBS and incubated with a donkey anti-mouse Alexa Fluor 568 (ThermoFisher Scientific, A10037) and donkey anti-rabbit Alexa Fluor 488 (ThermoFisher Scientific, A21206) secondary antibodies (1:400) for one hour in the dark
NAC is generally non-toxic and excess amounts are normally urinated out
At the desired time point, cells were fixed with 4% PFA for 45 min, permeabilized with 0.2% Triton X-100 (Sigma, 11332481001) diluted in phosphate-buffered saline (PBS) for 10 min and blocked with 1% bovine serum albumin (Sigma, cat
Prolonged or excessive use can lead to gastrointestinal issues, kidney damage, and cardiovascular risks
10.1074/jbc.274.17.11455 118 SavaskanN
Although research into their direct anti-cancer effect is limited, they may be valuable as part of an integrated approach to cancer treatment in consultation with your practitioner