The next day, cells were treated with RSL3 or CHP, as indicated in the figure legends
thaliana MS isoforms have a variable number of cysteine residues, but only three of them are conserved, including the two zinc-binding cysteines (Figure 4 and Supplementary Table 5)
Researchers exploring lyophilized vs liquid peptides or other formulation types should prioritize sourcing from regulated entities within their country
Subsequently, 20 L of the supernatant was transferred into a 96-well black lightproof plate, and then 100 L of ATP detection reagent was added to each well for 5 min
Promega) (for AMYRs) or coelenterazine h (Nanolight Technologies, Pinetop, AZ) (for CTR) was then added to cells at a final concentration of 1:1000 or 5 M, respectively, and incubated for a further 1015 min
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