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Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)
May cause mild nausea or dizziness in some users
If the powder doesnt dissolve immediately with gentle swirling, refrigerating the vial for 15 to 30 minutes and then swirling again is a far safer approach
KPV A potent anti-inflammatory peptide that calms overactive immune pathways, supports gut healing, and helps balance hormones
4.8 Caspase-Glo 3/7 assay Caspases 3 and 7 activation were measured using Caspase-Glo 3/7 (Promega)
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