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Measurement of GSH Medium was collected for the determination of extracellular total GSH level and cells were homogenized in 5% 5-sulfosalicylic acid dihydrate (SSA, Sigma-Aldrich, MO, USA) on ice

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Mitochondrial problems were present as early as P15 (postnatal day 15) 17 , and they were also observed in the upper motor neurons (UMNs) of a broad spectrum of ALS patients with TDP-43 pathology 16