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The extrapineal biosynthetic locations include the retina, harderian gland (complementary to the lacrimal), bone marrow, skin, cerebellum, cells of the immune system (lymphocytes), and particularly cells of the gastrointestinal tract that produce serotonin

After observing food intake the researchers examined how those changes affected body weight during the 12 hour observation period

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The latter oxidation reactions are essentially irreversible and hence lead to protein inactivation