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Two independent controlled studies using the same dose concluded the effect was of little clinical significance and not likely to be of major therapeutic benefit. Because the negative studies matched the positive studies dose, the failure cannot be explained by underdosing

Around D20 neurons were dissociated with Accutase and plated on matrigel-coated glass cover slips or in 35-mm culture wells with glass bottom

No matter what option you choose, you should explore these options with healthcare providers: this is essential to determine the most effective course of action

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They simply stop working, and the most common interpretation is that the protocol itself is not effective