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On the surface, these results do not appear to align with the predicted immunogenicity shown in the quadrant plot ( Figure 5 )

It becomes biologically inactive regardless of dose calculation accuracy

In addition, the FDA determined Enzeevu to be interchangeable with its reference medicine, Regenerons Eylea, and the drug holds unexpired exclusivity for first interchangeable biosimilar products

10.1021/jf0502698

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Thereafter, D-Hanks was used to rinse the samples, which were inoculated with primary antibodies against pAMPK (1:300) and CPT1 (1:300) at cold storage overnight