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After the treatment period, cells were fixed in 4% paraformaldehyde, permeabilized using 90% methanol, and incubated at room temperature for 1 h with primary antibodies against GPX4 and SLC7A11 (1:2000)
Since immune cells also require substantial proliferation to function after activation, there has been progressively increasing research focusing on glutaminolysis in immune cells in recent years
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Therefore, the treatment strategies during phase II follow the treatments employed during phase I: babies should be treated with anti-inflammatory, neuroprotective, or nerve regenerating agents (e.g., nerve growth factor), and stem cell transplantation
