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Total RNA was isolated from cells by hot phenol extraction ( Calculations For second-order reactions, the half-life of a reactant (with the second reactant concentration remaining constant) is described by a simple exponential equation: Ln(0.5) = k[R 2 ]t 1.2 , where k is the rate constant, [R 2 ] represents the concentration of the second reactant, and t 1.2 is the half-life