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inhibitors of cathepsin B, a proteinase linked to the inflammatory demyelination of MS

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Freezing the reconstituted solution is also an option for longer-term storage, but this comes with its own set of rulesnamely, avoiding repeated freeze-thaw cycles which can fracture the peptide structure

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Followed by SDS-PAGE electrophoresis, the transfer of proteins was conducted onto PVDF (Millipore) membranes (Solebio, Beijing, China) and immunocontainment experiments were performed after the membrane transfer was completed, the primary antibody was added and incubated at 4 C overnight with slow shaking