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For the assembly of DDB1-DET1-DDA1-COP1 complex, His-tagged DDB1, TSF-tagged human DET1, GST-tagged COP1 and untagged DDA1 were co-transfected into Expi293F cells as previously described

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a specific increase of fibroblast-specific GPX3 expression or a spatial shift from apically secreted soluble GPX3 to interstitial matrix-associated GPX3, reminiscent of what has been observed in a mouse model of influenza pneumonia 33

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