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These immunosuppressive proteins contribute to the establishment of an immunologically suppressive tumor microenvironment and are associated with poorer survival outcomes in preclinical glioblastoma models

The protein was eluted in three steps, first with 5 ml of elution buffer containing 50 mM imidazole (50 mM NaH 2 PO 4 , 300 mM NaCl and 50 mM imidazole, pH 8.0) and then with 5 ml of elution buffer containing 100 mM imidazole and finally with 5 ml of elution buffer containing 500 mM of imidazole

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