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Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h

Nausea, dehydration, electrolyte imbalances, and micronutrient deficiencies are more common with combined therapy

Pregnant or breastfeeding women, individuals with kidney or liver disorders, and those allergic to glutathione should avoid this treatment

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These effects can be frustrating for people who need prednisone for medical reasons but are concerned about gaining weight