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For the generation of Go-Nt 7 -FM 4 -GFP and MGNC-Nt 7 -FM 4 -GFP, the FM 4 sequence was PCR-amplified from the GFP-FM 4 -hGH plasmid (Andrew Peden, University of Sheffield), cut with AgeI/PstI and ligated in frame into the same sites of Go-Nt 7 -GFP and MGNC-Nt 7 -FM 4 -GFP