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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

Glutathione binds to them and renders them harmless

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A clear, colorless and odorless oily liquid that works as a fast-spreading emollient with a dry skin feel

441,442,443,444 Compared with individual functional peptides, multifunctional peptide carriers considerably improve transfection efficiency and have demonstrated outstanding potential in clinical applications

Site-specific pka determination of selenocysteine residues in selenovasopressin by using 77Se NMR spectroscopy