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After TMB substrate solution is added, only those wells that contain Human FGF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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The day before the assay, Seahorse probe plates were prepared by adding 200 l distilled water, and calibration buffer was added to a 50 ml Falcon tube

doi: 10.4065/77.8.754

For example, NRF2 activation was found to protect cells against ionizing radiation toxicity and confer radioresistance [98]