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However, causality is complex and not fully established in all cases

Transwell migration and invasion assays were performed using a 24-well transwell chamber with matrix gel as described previously [35]

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The storage requirements are nearly identical: refrigeration at 2 to 8 degrees Celsius, room temperature tolerance up to 21 days, and the same prohibition against freezing

Following a PBS rinse, the sections were incubated for one hour at room temperature with biotinylated secondary antibody (Goat Anti-Rabbit IgG, ab6721) and then for ten minutes with streptavidin

That makes it difficult to compare one brand to another or even to measure the effects of a single brand