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For the thickness of the retinal tissue, internal plexiform layer, and internal nuclear layer, the measurements (ISSA program (VAMSTEC, Zagreb, Croatia) from the area of maximal tissue damage detected using semi-serial sections, five high-power fields were randomly selected for analysis, were made with an ocular micrometer at 20 magnification within 0.5 mm from the optic disc

The strongest evidence we currently have relates to gut motility, not IBS specifically

All other DIA-NN settings were left default, using RT-dependent cross-run normalization and filtering the output at 1% FDR

Sleep quality: Weight loss and metabolic changes can sometimes temporarily disrupt sleep patterns, indirectly worsening daytime tiredness

Healthcare professionals should discuss realistic expectations and individualised treatment goals, focusing on health improvements beyond the number on the scales, such as improved glycaemic control, blood pressure reduction, and enhanced quality of life