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Subsequent processing was performed as described in the Sample preparation for total proteome and analysis of ubiquitylated peptides section, with the only modification being the use of 10% formic acid instead of 10% trifluoroacetic acid (TFA) for peptide acidification prior to desalting

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[DOI] [PMC free article] [PubMed] [Google Scholar] 65.Mulcahy RT, Untawale S, Gipp JJ

Bibcode:2025ACSNa..1932108B

Upon reperfusion, the sudden influx of oxygen leads to a burst of ROS production, exacerbating cellular damage

doi: 10.1124/jpet.108.142208