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For mitochondrial function assay, methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay was performed

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Emerging research suggests GLP-1 receptor agonists may reduce inflammatory markers such as C-reactive protein and pro-inflammatory cytokines, though dedicated clinical trials examining low-dose regimens specifically for inflammation are lacking

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We also detected the key genes involved in the apoptosis pathway, including BCL2 Apoptosis Regulator ( Bcl2 ), BCL2 Like 1 ( Bcl2l1 ), BCL2 Associated Agonist Of Cell Death ( Bad ), Caspase 3 ( Casp3 ), and Caspase 9 ( Casp9 )