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Our data suggest that a moderate dose of ibuprofen treatment for 7 days significantly altered oxylipins related to different metabolic pathways and revealed significant sex specific differences in livers from ibuprofen treated males and females relative to their vehicle controls

[PubMed] [Google Scholar] 94.Kolesnichenko L.S., Kulinski V.I., Shprakh V.V., Bardymov V.V., Verlan N.V., Gubina L.P., Pensionerova G.A., Sergeeva M.P., Stanevich L.M., Filippova G.T

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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

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K., Echeverri, C