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Spiking experiments into the plasma fraction prior to extraction showed that enzymes catalyzing the conversion of NADH to NAD+ are localized in plasma, while those affecting NAD + , NADP + and NADPH are associated with the cellular fraction (Supplementary Table 1, Sample Type 4)

The RUVs method, specifically, uses factor analysis on the differences between replicate groups of samples for estimating factors of unwanted variation, which can then be included in a linear model

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