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The membranes were then incubated with horseradish peroxidase-linked secondary antibodies (Cell Signaling Technology) and the signals were visualized by enhanced chemiluminescence using an Amersham Imager 600 (GE Healthcare)

Glutathione protects cells by neutralizing free radicals and recycling other antioxidants like vitamin C and E

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You have simply created a lower concentration than intended, which means you will draw a larger volume per dose

J Cereb Blood Flow Metab 28(10):16521655 Powers SK, Jackson MJ (2008) Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production

In general, liver biopsy is performed with a special needle and very effective local anesthesia, along with some sedation