Pre-seed culture was made by inoculating 0.2% (v/v) working cell bank of E
After the reaction, the acetonitrile was removed by rotary evaporation, an appropriate amount of ethyl acetate was added to dissolve the product, and 4% NaHCO was used for each
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The nucleotide sequence of semapeptide (nucleotide sequence) is: GAG GGC ACC TTC ACC AGC GAC GTT AGC AGC TAT CTG GAG GGC CAG GCG GCG AAA GAA TTT ATT GCG TGG CTG GTT CGT GGT CGT GGC
Procd de prparation selon l'une quelconque des revendications 1 3, dans lequel le couplage dans la synthse fragmentaire de la chane latrale de Lys 26 est ralis par condensation avec DIC+A ou B+A+C jusqu' ce que la rsine soit dtecte comme tant transparente avec de la ninhydrine, dans lequel A est choisi parmi du HOBt ou du HOAt, B est choisi parmi du HBTU, du HATU, du TBTU, du PyAOP, ou du PyBOP, C est choisi parmi de la DIPEA ou du TMP
In an aspect, expression vector that is used in the process of the present invention can be commercially available expression vectors or custom designed vectors selected from pET vectors, pd451sR and the like
