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The thirty rats were divided into the following four groups control (C), whey protein concentrate-treated control (C + WPC), methotrexate administration (MTX) and whey protein concentrate-treated methotrexate administered (MTX + WPC) groups

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Similarly, both in vivo feeding with a choline-rich diet and in vitro trimethylamine-N-oxidase (TMAO) stimulation increased TMAO-dependent NLRP3 inflammasome activation and expression of IL-1, IL6, TNF-, CXCL9, and CXCL10, supporting that NLRP3 is a key proteolytic activator in the macrophage response to a high choline and TMAO production ( Figure 3 )