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NFS1 degrades cysteine and releases sulphide, which can be used to generate ironsulphur (FeS) clusters, 138 versatile co-factors that carry out electron transfer, catalysis and afford structural stability

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The superoxide generated is detoxified by superoxide dismutase into H 2 O and H 2 O 2 , which is further detoxified by catalase and glutathione peroxidase (with the help of glutathione) into H 2 O and O 2

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Glutathione affinity chromatography for the purification of GST-fusion proteins